Cytomorphologic visualization of circulating tumor cells in urinary bladder cancer patients using ScreenCell™ technology: Potential as a simple cytology test

Author:

Jahan Meriyam1,Mittal Ankur2,Rao Shalinee1,Kishore Sanjeev1,Singh Ashok1,Jadli Mohit3,Paul Pranoy3ORCID,Iqbal Asif4,Chowdhury Nilotpal13ORCID

Affiliation:

1. Department of Pathology and Laboratory Medicine All India Institute of Medical Sciences Rishikesh India

2. Department of Urology All India Institute of Medical Sciences Rishikesh India

3. Multidisciplinary Research Unit All India Institute of Medical Sciences Rishikesh India

4. Healthica Path Labs New Delhi India

Abstract

AbstractCirculating tumor cells (CTC) are a recent technique which is a potentially important prognostic factor in many solid tumors. There are many techniques of detecting CTCs, but they usually implement costly techniques like EpCAM targeted detection, fluorescence‐based diagnosis, or magnetic bead based positive or negative selection. The diagnostic utility of simple cytomorphological diagnosis after routine staining of CTCs have been rarely studied. We aimed to detect CTCs in 24 patients clinically suspected to have Urinary Bladder Cancer using a simple but efficient patented filtration technology (ScreenCell™), followed by optical microscopic visualization after routine May‐Grunwald‐Giemsa (MGG) staining. The detected CTCs were then tested for association with the histologic type, lamina propria invasion, deep muscle invasion and the T‐stage. Out of the 24 patients tested, one was found to have papilloma, nine had low grade urothelial carcinoma, 13 had high grade urothelial carcinoma and one had poorly differentiated adenocarcinoma. Of these, two LGUC, eight HGUC and one adenocarcinoma had detectable CTC. Presence of CTCs had a statistically significant association with Lamina propria invasion (P = .006) and T‐stage (P = .02), and a trend toward significance for differentiating LGUC from HGUC (P = .10). These results suggest that cytomorphological detection of CTC is likely to be clinically useful in diagnosis and prognostication of urinary blader cancers. These findings need to be confirmed on studies with larger sample sizes.

Publisher

Wiley

Subject

General Medicine,Histology,Pathology and Forensic Medicine

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