CircWHSC1 serves as a prognostic biomarker and promotes malignant progression of non‐small‐cell lung cancer via miR‐590‐5p/SOX5 axis

Abstract

AbstractDysregulated circWHSC1 has been shown to play potential roles in diverse cancer types, including ovarian cancer, endometrial cancer and hepatocellular carcinoma (HCC). The objective of this study was to investigate its expression, underlying role and regulatory mechanism in non‐small‐cell lung cancer (NSCLC). The expression of circWHSC1 was determined by real‐time PCR. After knockdown of circWHSC1 expression in NSCLC cells, the proliferation, migration, and invasion were detected using CCK‐8, colony formation, and Transwell assays, and the effects of circWHSC1 on NSCLC tumorigenesis in vivo was also investigated. With the help of luciferase reporter and pull‐down assays, we further explored the downstream mechanism of circWHSC1 in NSCLC cells. CircWHSC1 was highly expressed in NSCLC tissues and cell lines. The inhibition of circWHSC1 suppressed the malignant properties of NSCLC cells, as evidenced by the reduction of proliferation, migration and invasion. CircWHSC1 sponged miR‐590‐5p and functioned as an oncogene in NSCLC by increasing sex determining region Y‐boxprotein 5 (SOX5) expression. CircWHSC1 may contribute to the oncogenicity of NSCLC via the regulation of miR‐590‐5p/SOX5 axis, which might be a novel therapeutic target in NSCLC.