Affiliation:
1. Babraham Institute, Babraham Research Campus Cambridge UK
Abstract
Extraction protocols and liquid chromatography coupled with mass spectrometry (LC–MS) and tandem mass spectrometry (LC–MS/MS) methods for the measurement of four lipid categories, namely glycerophospholipids, glycerolipids, sphingolipids and sterol lipids in human plasma, are described here. Step‐by‐step instructions are provided for the liquid–liquid extraction methods, including solution preparation and the non‐endogenous lipid internal standards used. All instrumental conditions, chromatography columns and solutions required for the LC–MS and LC–MS/MS methods are also provided in detail.
Subject
Organic Chemistry,Spectroscopy,Analytical Chemistry