Continuous Preparative Application of Amine Transaminase‐Catalyzed Reactions with Integrated Crystallization

Author:

Tiedemann Sven12,Neuburger Jan Eric1,Gazizova Alina3,von Langermann Jan12

Affiliation:

1. University of Rostock Institute of Chemistry Biocatalytic Synthesis Group Albert-Einstein-Str. 3a 18059 Rostock Germany

2. Otto-von-Guericke University Magdeburg Institute of Chemistry Biocatalysis Group Universitätsplatz 2 39106 Magdeburg Germany

3. University of Rostock Institute of Chemistry Technical Chemistry Albert-Einstein-Str. 3a 18059 Rostock Germany

Abstract

AbstractTransaminases are valuable catalysts in the synthesis of chiral amines. However, secondary techniques are typically required to shift the reaction equilibrium to the product side. This study focuses on the in situ product crystallization, specifically at preparative synthetic scale as a non‐synthetic tool for a selection of aromatic and aliphatic substrates using the transaminase from Silicibacter pomeroyi. Herein the technique is implemented in a simplified manner to facilitate in situ product removal and downstream‐processing in a combined crystallization step. After a series of optimizations, product concentrations of >0.9 mol/L and >99 % e.e. (S) were obtained implementing a repetitive batch reaction approach using a universal carboxylate as anion.

Funder

Deutsche Forschungsgemeinschaft

Bundesministerium für Wirtschaft und Klimaschutz

Publisher

Wiley

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