Highly sensitive detection of mutations in CHO cell recombinant DNA using multi-parallel single molecule real-time DNA sequencing
Author:
Affiliation:
1. Department of Chemical and Biological Engineering; University of Sheffield; Sheffield UK
2. Cell Line Development; BioTherapeutic Pharmaceutical Sciences; Pfizer Inc; Andover Massachusetts
3. Pacific Biosciences; Menlo Park California
Funder
Engineering and Physical Sciences Research Council
Pfizer
Publisher
Wiley
Subject
Applied Microbiology and Biotechnology,Bioengineering,Biotechnology
Reference68 articles.
1. What's fueling the biotech engine-2012 to 2013;Aggarwal;Nature Biotechnology,2014
2. Utilization of Site-Specific Recombination in Biopharmaceutical Production;Ahmadi;Iranian Biomedical Journal,2016
3. Modified Hirt procedure for rapid purification of extrachromosomal DNA from mammalian cells;Arad;BioTechniques,1998
4. PCR-induced transitions are the major source of error in cleaned ultra-deep pyrosequencing data;Brodin;PLoS ONE,2013
5. Contribution of intra- and interhost dynamics to norovirus evolution;Bull;Journal of Virology,2012
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