Affiliation:
1. Fischell Department of Bioengineering University of Maryland College Park Maryland USA
2. Institute of Bioscience and Biotechnology Research University of Maryland College Park Maryland USA
3. Fischell Institute of Biomedical Devices University of Maryland College Park Maryland USA
4. Lawrence Livermore National Laboratory Biosciences and Biotechnology Division Livermore California USA
Abstract
AbstractTo probe signal propagation and genetic actuation in microbial consortia, we have coopted the components of both redox and quorum sensing (QS) signaling into a communication network for guiding composition by “programming” cell lysis. Here, we use an electrode to generate hydrogen peroxide as a redox cue that determines consortia composition. The oxidative stress regulon of Escherichia coli, OxyR, is employed to receive and transform this signal into a QS signal that coordinates the lysis of a subpopulation of cells. We examine a suite of information transfer modalities including “monoculture” and “transmitter‐receiver” models, as well as a series of genetic circuits that introduce time‐delays for altering information relay, thereby expanding design space. A simple mathematical model aids in developing communication schemes that accommodate the transient nature of redox signals and the “collective” attributes of QS signals. We suggest this platform methodology will be useful in understanding and controlling synthetic microbial consortia for a variety of applications, including biomanufacturing and biocontainment.
Funder
U.S. Department of Energy
Defense Threat Reduction Agency
Lawrence Livermore National Laboratory
Subject
Applied Microbiology and Biotechnology,Bioengineering,Biotechnology
Cited by
4 articles.
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