Biofiltration Reactivation Kinetics of a Cyclobio Fluidized Sand Filter in a Warmwater Recirculating Aquaculture System

Abstract

AbstractThe ability of a commercial‐scale Cyclobio fluidized sand filter was evaluated for ammonia and nitrite removal by dosing with ammonia chloride, NH4Cl, as part of the water treatment unit for the U.S. Department of Agriculture Agricultural Research Service Warmwater Aquaculture Research Unit's (WARU) catfish hatchery in Stoneville, Mississippi, USA. Treatment system water was recirculated through the Cyclobio filter for several months without any feed inputs; thus, our objective was to investigate how quickly a full‐scale Cyclobio fluidized sand filter could be reactivated with NH4Cl substrate dosing in preparation for the upcoming spawning season. A second objective of the study was to examine the decline of the filter's nitrification ability when the supplementary NH4Cl substrate dosings were removed or limited. The effects of daily and weekly substrate additions were evaluated over a 14‐week winter period, with the system water temperature ranging between 20° and 23°C. Total ammonia nitrogen (TAN) concentration decreased within hours of initial dosing. After approximately a 2‐h lag period, the nitrite–nitrogen concentration peaked and then decreased. The rate of TAN nitrification followed a simple first‐order reaction kinetics and increased over time during the 4 weeks of daily NH4Cl substrate dosing. The return to a weekly substrate dosage resulted in a decrease in the kinetic reaction rate, but adequate to maintain the filter during periods of system inactivity. The once‐daily substrate dosage was consumed within hours of the initial dosing and was sufficient for current system loading.