An improved method for primary culture of normal cervical epithelial cells and establishment of cell model in vitro with HPV-16 E6 gene by lentivirus

Author:

Fan Tingting1,Li Xiaofu1,Li Ya1,Zhi Yanfang1,Rong Shouhua1,Cheng Guomei2,Zhang Xiaoan3ORCID

Affiliation:

1. Department of Cytopathology; The Third Affiliated Hospital of Zhengzhou University; Zhengzhou China

2. Department of Obstetrics and Gynecology; The Third Affiliated Hospital of Zhengzhou University; Zhengzhou China

3. Department of Imaging; The Third Affiliated Hospital of Zhengzhou University; Zhengzhou China

Funder

Science and Technology Department of Henan (China)

Publisher

Wiley

Subject

Cell Biology,Clinical Biochemistry,Physiology

Reference25 articles.

1. Cervical epithelial cells transduced with the papillomavirus E6/E7 oncogenes maintain stable levels of oncoprotein expression but exhibit progressive, major increases in hTERT gene expression and telomerase activity;Baege;American Journal of Pathology,2002

2. Optimal lentivirus production and cell culture conditions necessary to successfully transduce primary human bronchial epithelial cells;Baumlin-Schmid;Journal of Visualized Experiments,2016

3. Calcium-regulated differentiation of normal human epidermal keratinocytes in chemically defined clonal culture and serum-free serial culture;Boyce;Journal of Investigative Dermatology,1983

4. Characterization and functional analysis of the expression of intercellular adhesion molecule-1 in human papillomavirus-related disease of cervical keratinocytes;Coleman;American Journal of Pathology,1993

5. The phenotype of in vitro reconstituted normal human oral epithelium is essentially determined by culture medium;Costea;Journal of Oral Pathology & Medicine,2005

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