Nondestructive Evaluation of Metal Bioaccumulation and Biochemical Biomarkers in Blood of Broad‐Snouted Caiman (<i>Caiman latirostris</i>) from Northeastern Brasil-Reference-Cited by-同舟云学术

Nondestructive Evaluation of Metal Bioaccumulation and Biochemical Biomarkers in Blood of Broad‐Snouted Caiman (Caiman latirostris) from Northeastern Brasil

Published:2024-02-27 Issue:4 Volume:43 Page:878-895
ISSN:0730-7268
Container-title:Environmental Toxicology and Chemistry
language:en
Short-container-title:Enviro Toxic and Chemistry

Author:

dos Santos Rayssa Lima¹²³^ORCID,Mariz Jr. Célio Freire¹³,Mascarenhas‐Júnior Paulo Braga¹²,Barboza Rafael Sá Leitão²⁴^ORCID,dos Santos Ednilza Maranhão⁴,de Sousa Correia Jozélia Maria⁴,de Carvalho Paulo Sérgio Martins³

Affiliation:

1. Programa de Pós‐Graduação em Biologia Animal Universidade Federal de Pernambuco Recife Brasil

2. Laboratório Interdisciplinar de Anfibios e Répteis, Universidade Federal de Pernambuco Recife Brasil

3. Laboratório de Ecotoxicologia Aquática, Universidade Federal de Pernambuco Recife Brasil

4. Programa de Pós‐Graduação em Biodiversidade Universidade Federal Rural de Pernambuco Recife Brasil

Abstract

AbstractStudies on the bioaccumulation and toxicity of contaminants in Crocodylians are scarce. We evaluated alterations in concentrations of the nondestructive biomarkers butyrylcholinesterase (BChE), glutathione‐S‐transferase (GST), superoxide dismutase (SOD), and reduced glutathione (GSH), together with bioaccumulation of the metals iron (Fe), copper (Cu), zinc (Zn), manganese (Mn), chronium (Cr), aluminium (Al), and lead (Pb) in Caiman latirostris captured in Tapacurá Reservoir (TR; São Lourenço da Mata, Pernambuco, Brasil), in urbanized areas of Pernambuco State (UA; Brasil) and from the AME Brasil caiman farm (AF; Marechal Deodoro, Alagoas, Brasil); the latter was used as a potential reference with low levels of contamination. For metal analysis, 500 µL of blood was digested in 65% HNO3 and 30% H2O2. The samples were analyzed by inductively coupled plasma–optical emission spectrometry. For analysis of biomarkers, an aliquot of blood was centrifuged to obtain plasma in which biochemical assays were performed. Blood concentrations of metals analyzed in animals from AF were lower compared with TR and UA, confirming that animals from the caiman farm could be used as references with low levels of contamination. Iron, Cu, Mn, Al, and Pb exceeded toxic levels for other vertebrates in animals from TR and UA. Butyrylcholinesterase activity showed significant reduction in adults from UA and TR compared with AF. An increase in the activity of GST and GSH, in adults of TR and UA in relation to AF, was verified. Superoxide dismutase activity showed a significant reduction in adults of TR in relation to AF, and the concentrations of Cu and Mn were negatively correlated with SOD activity. Animals from UA and TR showed greater concentrations of the analyzed metals compared with reference animals, and changes in biomarkers were seen, confirming the potential of these nondestructive chemical and biological parameters in blood of C. latirostris for biomonitoring of pollution. Environ Toxicol Chem 2024;43:878–895. © 2024 SETAC

Publisher

Wiley

Link

https://setac.onlinelibrary.wiley.com/doi/pdf/10.1002/etc.5823

Reference106 articles.

1. Araújo Freitas J. B. Cabral J. J. dS. P. Luiz A. Paiva R. Albuquerque T. B. V. &Nascimento Silva N. B.(2018).Qualidade de água em um trecho urbano do rio Beberibe na Região Metropolitana do RecifeXII ENAU–Encontro Nacional de Águas Urbanas Maceió–AL.

2. Contamination and Toxicity of Surface Waters Along Rural and Urban Regions of the Capibaribe River in Tropical Northeastern Brazil

3. Distribution and enrichment of heavy metals at the Tapacurá River basin, northeastern Brazil

4. Biotransformation and Oxidative Stress Responses in Captive Nile Crocodile (Crocodylus niloticus) Exposed to Organic Contaminants from the Natural Environment in South Africa

5. Diabetes mellitus and oxidative stress—A concise review