Affiliation:
1. Department of Biosciences and Biomedical Engineering Indian Institute of Technology Indore Indore Madhya Pradesh India
2. Institute of Epigenetics Fox Chase Cancer Center Philadelphia Pennsylvania USA
3. Department of Chemistry University of Pennsylvania Philadelphia Pennsylvania USA
4. Materials and Device Laboratory, Department of Physics Indian Institute of Technology Indore Indore Madhya Pradesh India
5. Department of Bioscience and Bioengineering Indian Institute of Technology Jodhpur Karwar Rajasthan India
6. Centre for Rural Development and Technology Indian Institute of Technology Indore Indore Madhya Pradesh India
Abstract
AbstractHelicobacter pylori and Epstein–Barr Virus (EBV) are Group 1 carcinogens that can enhance gastric cancer progression. Bioactive substances extracted from plants can be effective therapeutic agents in cancer treatment. For example, Withania somnifera extract‐WSE reduces the Gankyrin oncoprotein, which is upregulated in the presence of H. pylori and EBV. The various biochemical and metabolic changes upon 24 hrs post‐infection followed by W. somnifera extract (WSE) treatment on gastric epithelial cells (AGS) can be studied using spectroscopic techniques. In the biomedical sciences, Raman and NMR spectroscopy have been extensively employed to interpret cellular alterations contributing to the onset of infection and the severity of gastric cancer. More specifically, alterations in cellular biochemical homeostasis are linked to the moieties of cholesterol, collagen, choline, carbohydrate, lipids, tyrosine, and phenylalanine. Further, we have found significantly elevated FWHM for carbohydrates, tumor associated protein, collagen, cholesterol, and cholesterol ester in the co‐infection model. We also looked into the potential correlation between these molecules using molecular network analysis and found several related factors that can be modulated through biomolecular levels. These molecules are crucial in several physiological functions, including cell division, cell proliferation, apoptosis, necrosis, cell migration, and lipid transport. Our study paves the pathway to study H. pylori and EBV co‐infection in human gastric epithelial cells and the therapeutic interventions of WSE in this scenario and highlights specific biomolecular alterations, which can be focused for further mechanistic investigations.
Funder
CSIR - Institute of Microbial Technology
Douglas Bomford Trust
University Grants Commission - Sri Lanka
Subject
Spectroscopy,General Materials Science