CRISPR/Cas9 screen in human iPSC‐derived cortical neurons identifies NEK6 as a novel disease modifier of C9orf72 poly(PR) toxicity

Author:

Guo Wenting123ORCID,Wang Haibo45,Kumar Tharkeshwar Arun23,Couthouis Julien6,Braems Elke23,Masrori Pegah237,Van Schoor Evelien238,Fan Yannan1,Ahuja Karan1,Moisse Matthieu23,Jacquemyn Maarten9,Furtado Madeiro da Costa Rodrigo1,Gajjar Madhavsai1,Balusu Sriram3,Tricot Tine1,Fumagalli Laura23,Hersmus Nicole23,Janky Rekin's10,Impens Francis111213,Vanden Berghe Pieter14,Ho Ritchie15,Thal Dietmar Rudolf8,Vandenberghe Rik716,Hegde Muralidhar L.45,Chandran Siddharthan1718,De Strooper Bart317,Daelemans Dirk9,Van Damme Philip237,Van Den Bosch Ludo23ORCID,Verfaillie Catherine1

Affiliation:

1. Stem Cell Institute, Department of Devolpment and Regeneration, KU Leuven Leuven Belgium

2. Department of Neurosciences Experimental Neurology Laboratory of Neurobiology KU Leuven‐University of Leuven Leuven Belgium

3. VIB Center for Brain & Disease Research Leuven Belgium and Leuven Brain Institute (LBI) Leuven Belgium

4. Division of DNA Repair Research Department of Neurosurgery Center for Neuroregeneration Houston Methodist Research Institute Houston Texas USA

5. Department of Neuroscience Research at Neurological Surgery Weill Medical College New York New York USA

6. Department of Genetics Stanford University School of Medicine Stanford California USA

7. Department of Neurology University Hospitals Leuven Leuven Belgium

8. Laboratory of Neuropathology Department of Imaging and Pathology KU Leuven and Leuven Brain Institute (LBI) Leuven Belgium

9. KU Leuven Department of Microbiology Immunology and Transplantation Laboratory of Virology and Chemotherapy Rega Institute for Medical Research Leuven Belgium

10. VIB Nucleomics Core Leuven Belgium

11. VIB‐UGent Center for Medical Biotechnology Ghent Belgium

12. Department of Biomolecular Medicine Ghent University Ghent Belgium

13. VIB Proteomics Core Ghent Belgium

14. Translational Research Centre for Gastrointestinal Disorders (TARGID) KU Leuven—University of Leuven Leuven Belgium

15. Center for Neural Science and Medicine, Board of Governors Regenerative Medicine Institute, Departments of Biomedical Sciences and Neurology, Cedars‐Sinai Medical Center Los Angeles California USA

16. KU Leuven‐Laboratory for Cognitive Neurology Department of Neurosciences Leuven Brain Institute Leuven Belgium

17. UK‐Dementia Research Institute at University College London London UK

18. Centre for Clinical Brain Sciences University of Edinburgh Edinburgh UK

Funder

KU Leuven

Fonds Wetenschappelijk Onderzoek

National Institute of Neurological Disorders and Stroke

Houston Methodist Research Institute

Publisher

Wiley

Subject

Psychiatry and Mental health,Cellular and Molecular Neuroscience,Geriatrics and Gerontology,Neurology (clinical),Developmental Neuroscience,Health Policy,Epidemiology

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1. Genome-scale CRISPR-Cas9 screening in stem cells: theories, applications and challenges;Stem Cell Research & Therapy;2024-07-19

2. Discovery platforms for RNA therapeutics;British Journal of Pharmacology;2024-05-17

3. Elevated α-synuclein levels inhibit mitophagic flux;npj Parkinson's Disease;2024-04-09

4. Induced Pluripotent Stem Cells in the Era of Precise Genome Editing;Current Stem Cell Research & Therapy;2024-04

5. CRISPRi: a way to integrate iPSC-derived neuronal models;Biochemical Society Transactions;2024-03-25

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