Affiliation:
1. School of Natural Sciences Macquarie University Sydney New South Wales Australia
2. Australian Research Council Industrial Transformation Training Centre for Facilitated Advancement of Australia's Bioactives (FAAB) Sydney New South Wales Australia
3. JASCO International Co., Ltd Hachioji Tokyo Japan
Abstract
AbstractMembranes are important sites of intermolecular interactions in biological systems. However, they present significant analytical challenges as they contain multiple analytes and are dynamic in nature. In this work, we show how a Jasco J‐1500 circular dichroism spectropolarimeter can be used with a microvolume Couette flow cell and appropriate cut‐off filters to measure excitation fluorescence detected linear dichroism (FDLD) of fluorophores embedded in liposomal membranes. The result is a spectrum that selectively probes the fluorophore(s) and eliminates the scattering that is apparent in the corresponding flow linear dichroism (LD) spectrum. The FDLD spectrum is opposite in sign from the LD spectrum with relative magnitudes modified by the quantum yields of the transitions. FDLD thus enables analyte orientations to be identified in a membrane. Data for a membrane peptide, gramicidin, and two aromatic analytes, anthracene and pyrene, are presented. Issues with the “leakage” of photons by the long pass filters used is also discussed.
Subject
Organic Chemistry,Spectroscopy,Drug Discovery,Pharmacology,Catalysis,Analytical Chemistry
Cited by
2 articles.
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