Split‐enzyme fragment as a single affinity tag that enables protein expression, purification, and functional assays
Author:
Affiliation:
1. Department of Pharmaceutics and Pharmaceutical ChemistryUniversity of UtahSalt Lake City Utah
2. Department of Biomedical EngineeringUniversity of UtahSalt Lake City Utah
3. Department of Medicinal ChemistryUniversity of UtahSalt Lake City Utah
Publisher
Wiley
Subject
Applied Microbiology and Biotechnology,Bioengineering,Biotechnology
Link
https://onlinelibrary.wiley.com/doi/pdf/10.1002/bit.26980
Reference24 articles.
1. Brunelle J. L. &Green R.(2014). Coomassie blue staining. InMethods in enzymology(Vol.541 pp.161–167). Elsevier: Netherlands.
2. NanoLuc Complementation Reporter Optimized for Accurate Measurement of Protein Interactions in Cells
3. A new set of highly efficient, tag-cleaving proteases for purifying recombinant proteins
4. Development of the designed ankyrin repeat protein (DARPin) G3 for HER2 molecular imaging
5. Engineered Luciferase Reporter from a Deep Sea Shrimp Utilizing a Novel Imidazopyrazinone Substrate
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