Two-Step Co-Immunoprecipitation (TIP)

Author:

Sciuto Maria Rita1,Coppola Valeria1,Iannolo Gioacchin2,De Maria Ruggero34,Haas Tobias L.34

Affiliation:

1. Department of Oncology and Molecular Medicine, Istituto Superiore di Sanità; Rome Italy

2. Regenerative Medicine and Biomedical Technologies Unit, Department of Laboratory Medicine and Advanced Biotechnologies, IRCCS-ISMETT; Palermo Italy

3. Institute of General Pathology, Università Cattolica del Sacro Cuore; Rome Italy

4. Fondazione Policlinico Universitario A. Gemelli IRCCS; Rome Italy

Funder

FIRB

Italian Ministry of Health

Ministero dell'istruzione, dell'Università e della Ricerca

Ministero della Salute

Publisher

Wiley

Subject

General Medicine

Reference30 articles.

1. Reproducibility crisis: Blame it on the antibodies;Baker;Nature,2015

2. High-throughput mapping of a dynamic signaling network in mammalian cells;Barrios-Rodiles;Science,2005

3. Identifying novel protein interactions: Proteomic methods, optimisation approaches and data analysis pipelines;Carneiro;Methods (San Diego, Calif.),2016

4. Mechanisms of T cell activation by the calcium ionophore ionomycin;Chatila;Journal of Immunology (Baltimore, Md.: 1950),1989

5. A novel strategy for the immunological tagging of cDNA constructs;Cravchik;Gene,1993

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