UV‐LASER adjuvant–surfactant‐facilitated delivery of mobile dsRNA to tomato plant vasculature and evidence of biological activity by gene knockdown in the potato psyllid

Author:

Thakre Neha12ORCID,Carver Megan1,Paredes‐Montero Jorge R34,Mondal Mosharrof1,Hu Jiahuai1,Saberi Esmaeil5,Ponvert Nathaniel1,Qureshi Jawwad A5ORCID,Brown Judith K1

Affiliation:

1. School of Plant Sciences The University of Arizona Tucson AZ USA

2. Division of Biological Sciences, Section of Cell and Developmental Biology University of California San Diego La Jolla CA USA

3. Biology Department Saginaw Valley State University University Center USA

4. Facultad de Ciencias de la Vida, Escuela Superior Politécnica del Litoral, ESPOL, Campus Gustavo Galindo Guayaquil Ecuador

5. Department of Entomology and Nematology, IFAS, Southwest Florida Research and Education Center University of Florida Immokalee FL USA

Abstract

AbstractBACKGROUNDDouble‐stranded RNA (dsRNA) biopesticides are of interest for the abatement of insect vectors of pathogenic bacteria such as ‘Candidatus Liberibacter’, which infects both its psyllid and plant hosts. Silencing of genes essential for psyllids, or for Liberibacter, is anticipated to lead to mortality or impeded bacterial multiplication. Foliar delivery is preferred for biopesticide application; however, the cuticle impedes dsRNA penetration into the vasculature. Here, conditions were established for wounding tomato leaves using ultraviolet light amplification by stimulated emissions of radiation (UV‐LASER) to promote dsRNA penetration into leaves and vasculature.RESULTSUV‐LASER treatment with application of select adjuvants/surfactants resulted in vascular delivery of 100‐, 300‐ and 600‐bp dsRNAs that, in general, were correlated with size. The 100‐bp dsRNA required no pretreatment, whereas 300‐ and 600‐bp dsRNAs entered the vasculature after UV‐LASER treatment only and UV‐LASER adjuvant/surfactant treatment, respectively. Of six adjuvant/surfactants evaluated, plant‐derived oil combined with an anionic organosilicon compound performed most optimally. Localization of dsRNAs in the tomato vasculature was documented using fluorometry and fluorescence confocal microscopy. The biological activity of in planta‐delivered dsRNA (200–250 bp) was determined by feeding third‐instar psyllids on tomato leaves post UV‐LASER adjuvant/surfactant treatment, with or without psyllid cdc42‐ and gelsolin dsRNAs. Gene knockdown was quantified by quantitative, real‐time polymerase chain reaction with reverse transcription (RT–qPCR) amplification. At 10 days post the ingestion‐access period, knockdown of cdc42 and gelsolin expression was 61% and 56%, respectively, indicating that the dsRNAs delivered to the tomato vasculature were mobile and biologically active.CONCLUSIONResults indicated that UV‐LASER adjuvant/surfactant treatments facilitated the delivery of mobile, biologically active dsRNA molecules to the plant vasculature. © 2023 The Authors. Pest Management Science published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry.

Publisher

Wiley

Subject

Insect Science,Agronomy and Crop Science,General Medicine

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