Soybean Trypsin Inhibitor Assay: The Sequence Effect of Adding Reagents, Factors Involved, and Mechanistic Explanations

Author:

Liu Keshun1ORCID

Affiliation:

1. Grain Chemistry and Utilization Laboratory, National Small Grains and Potato Germplasm Research UnitUnited States Department of Agriculture, Agricultural Research Service (USDA‐ARS) 1691 S. 2700 West, Aberdeen ID 83210 USA

Publisher

Wiley

Subject

Organic Chemistry,General Chemical Engineering

Reference25 articles.

1. American Association of Cereal Chemists International (AACCI). (2009)Approved methods of analysis. 11th ed. Method 22‐40.01 (formerly 71–10). Measurement of trypsin inhibitor activity of soy products—Spectrophotometric method. First approval November 7 1973; Reapproved November 3 1999. St. Paul. MN.

2. American Oil Chemists’ Society (AOCS). (2017)Official methods and recommended practices. 7th ed. Method Ba 12‐75. Trypsin inhibitor activity. First approval 1975; Reapproved 2017. Champaign IL.

3. On the mechanism of enzyme action. LXXIII. Studies on trypsins from beef, sheep and pig pancreas

4. Peptide bond cleavage on trypsin‐trypsin inhibitor complex formation;Finkenstadt W. R.;The Journal of Biological Chemistry,1965

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