Novel mandelic acid derivatives suppress virulence of Ralstonia solanacearum via type III secretion system

Author:

Guo Qiao‐Qiao1,Li Yu‐Zhen1,Shi Hua‐Bin2,Yi Ao‐Yun1,Xu Xiao‐Li3,Wang Hai‐Hong4,Deng Xin56,Wu Zhi‐Bing2,Cui Zi‐Ning1ORCID

Affiliation:

1. National Key Laboratory of Green Pesticide, Integrative Microbiology Research Center, Guangdong Province Key Laboratory of Microbial Signals and Disease Control, College of Plant Protection South China Agricultural University Guangzhou China

2. National Key Laboratory of Green Pesticide, Key Laboratory of Green Pesticide and Agricultural Bioengineering, Ministry of Education Center for R&D of Fine Chemicals of Guizhou University Guiyang China

3. Instrumental Analysis and Research Center South China Agricultural University Guangzhou China

4. Guangdong Provincial Key Laboratory of Protein Function and Regulation in Agricultural Organisms, College of Life Sciences South China Agricultural University Guangzhou China

5. Department of Biomedical Sciences City University of Hong Kong Kowloon Tong Hong Kong

6. Shenzhen Research Institute City University of Hong Kong Shenzhen China

Abstract

AbstractBACKGROUNDBacterial wilt induced by Ralstonia solanacearum is regarded as one of the most devastating diseases. However, excessive and repeated use of the same bactericides has resulted in development of bacterial resistance. Targeting bacterial virulence factors, such as type III secretion system (T3SS), without inhibiting bacterial growth is a possible assay to discover new antimicrobial agents.RESULTSIn this work, identifying new T3SS inhibitors, a series of mandelic acid derivatives with 2‐mercapto‐1,3,4‐thiazole moiety was synthesized. One of them, F‐24, inhibited the transcription of hrpY gene significantly. The presence of this compound obviously attenuated hypersensitive response (HR) without inhibiting bacterial growth of R. solanacearum. The transcription levels of those typical T3SS genes were reduced to various degrees. The test of the ability of F‐24 in protecting plants demonstrated that F‐24 protected tomato plants against bacterial wilt without restricting the multiplication of R. solanacearum. The mechanism of this T3SS inhibition is through the PhcR‐PhcA‐PrhG‐HrpB pathway.CONCULSIONThe screened F‐24 could inhibit R. solanacearum T3SS and showed better inhibitory activity than previously reported inhibitors without affecting the growth of the strain, and F‐24 is a compound with good potential in the control of R. solanacearum. © 2023 Society of Chemical Industry.

Funder

National Natural Science Foundation of China

Publisher

Wiley

Subject

Insect Science,Agronomy and Crop Science,General Medicine

Cited by 2 articles. 订阅此论文施引文献 订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献

同舟云学术

1.学者识别学者识别

2.学术分析学术分析

3.人才评估人才评估

"同舟云学术"是以全球学者为主线,采集、加工和组织学术论文而形成的新型学术文献查询和分析系统,可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容,当前同舟云学术共收录了国内外主流学术期刊6万余种,收集的期刊论文及会议论文总量共计约1.5亿篇,并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询!咨询电话:010-8811{复制后删除}0370

www.globalauthorid.com

TOP

Copyright © 2019-2024 北京同舟云网络信息技术有限公司
京公网安备11010802033243号  京ICP备18003416号-3