Affiliation:
1. Department of Biology University of Padova Padova Italy
2. Department of Chemistry and Biotechnology University of Kochi Kochi Japan
Abstract
AbstractBackgroundColonial ascidians are chordates known to reproduce both sexually and asexually. In the present study, we used the Mediterranean species Botryllus schlosseri and the Japanese Botryllus primigenus to investigate the possible role of tiar, ttp, and g3bp in the periodical renewal of the colonies, defined by generation changes or takeovers. In this scenario, the above genes, which codify key components for the formation of stress granules, storing specific mRNAs, can play a pivotal role, allowing the regulation of processes such as stress responses, cell proliferation, and stem cell development.ResultsWe characterized tiarx1, ttp, and g3bp2 sequences in B. schlosseri and B. primigenus. Then, we analyzed gene transcription by in situ hybridization in hemolymph cells and colony tissues, and we proceeded with quantification of the gene transcription by quantitative real‐time PCR, during the phases of the colonial blastogenetic cycle.ConclusionsOur results allowed us to assign a role in the development of the new colonial generations to the studied genes.
Funder
Department of Biology, University of Dayton
Cited by
1 articles.
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