Three-dimensional culture of buffalo granulosa cells in hanging drop mimics the preovulatory follicle stage
Author:
Affiliation:
1. Molecular Endocrinology, Functional Genomics and Systems Biology Lab, Animal Biochemistry Division; ICAR-National Dairy Research Institute; Karnal India
Funder
Department of Biotechnology , Ministry of Science and Technology
Publisher
Wiley
Subject
Cell Biology,Clinical Biochemistry,Physiology
Link
http://onlinelibrary.wiley.com/wol1/doi/10.1002/jcp.25909/fullpdf
Reference22 articles.
1. Increasing cell plating density mimics an early post-LH stage in cultured bovine granulosa cells;Baufeld;Cell and Tissue Research,2013
2. Rapid effects of LH on gene expression in the mural granulosa cells of mouse periovulatory follicles;Carletti;Reproduction,2009
3. In vitro culture of early secondary preantral follicles in hanging drop of ovarian cell-conditioned medium to obtain MII oocytes from outbred deer mice;Choi;Tissue Engineering Part A,2013
4. A simple hanging drop cell culture protocol for generation of 3D spheroids;Foty;The Journal of Visualized Experiments,2011
5. Development of a long-term bovine granulosa cell culture system: Induction and maintenance of estradiol production, response to follicle-stimulating hormone, and morphological characteristics;Gutierrez;Biology of Reproduction,1997
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3. Three‐dimensional culture of rat ovarian granulosa cells shows increased SPP1 and FGF7 expression through the PI3K/Akt pathway;Cell Biology International;2023-01-26
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