Affiliation:
1. Department of Oncology The First Affiliated Hospital of Zhengzhou University Zhengzhou China
2. Tsinghua Shenzhen International Graduate School (SIGS) Tsinghua University Shenzhen China
Abstract
AbstractPatient‐derived organoids are gaining incremental popularity in both basic sciences and translational applications toward precision medicine and revolutionized drug discovery. However, for tumor organoids, challenges remain in low rates of organoid growth and tumor cell purity, that is, recapitulation of tumor phenotypes in constructed organoids. Here, we report a method of microfluidic droplet encapsulation that provides structural guidance for tumor cell growth and organization, where they develop into tumor organoids with high purity and high rates of modeling success, as compared to the classical organoid modeling method, that is, non‐engineered organoids. The modeling efficacy and organoid quality are examined in patient‐derived samples, covering esophagus, lung and colorectal cancer tissues, all proving significance in droplet‐engineered organoids, as demonstrated by histological examinations.
Funder
Education Department of Henan Province
National Natural Science Foundation of China
Science Fund for Distinguished Young Scholars of Guangdong Province
Science, Technology and Innovation Commission of Shenzhen Municipality