Off‐target glycans encountered along the synthetic biology route toward humanized N ‐glycans in Pichia pastoris

Author:

Laukens Bram12,Jacobs Pieter P.12,Geysens Katelijne3,Martins Jose3,De Wachter Charlot12,Ameloot Paul12,Morelle Willy4,Haustraete Jurgen5,Renauld Jean‐Christophe6,Samyn Bart2,Contreras Roland7,Devos Simon12ORCID,Callewaert Nico12

Affiliation:

1. VIB‐UGent Center for Medical BiotechnologyTechnologieparkZwijnaarde Belgium

2. Department of Biochemistry and MicrobiologyGhent UniversityGhent Belgium

3. NMR and Structural Analysis Unit, Department of Organic Chemistry and Macromolecular ChemistryGhent UniversityGhent Belgium

4. Glycobiologie Structurale et FonctionnelleUniversité des Sciences et Technologies de Lille 1Villeneuve d'Ascq France

5. Protein Service FacilityVIBGhent Belgium

6. Ludwig Institute for Cancer Research and Experimental Medicine UnitUniversité Catholique de LouvainBrussels Belgium

7. Department for Biomedical Molecular BiologyGhent UniversityGhent Belgium

Funder

Fonds Wetenschappelijk Onderzoek

Agentschap voor Innovatie door Wetenschap en Technologie

H2020 European Research Council

Publisher

Wiley

Subject

Applied Microbiology and Biotechnology,Bioengineering,Biotechnology

Reference37 articles.

1. N-glycan structures: recognition and processing in the ER

2. Acinar Cells of the Pancreas Are a Target of Interleukin-22

3. Glycosylation of Pichia pastoris‐derived proteins;Bretthauer R. K.;Biotechnology and Applied Biochemistry,1999

4. A simple and rapid method for the permethylation of carbohydrates

5. Transformation;Cregg J. M.;Methods in Molecular Biology,1998

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