Gene Editing to Generate Versatile Human Pluripotent Stem Cell Reporter Lines for Analysis of Differentiation and Lineage Tracing-Reference-Cited by-同舟云学术

Gene Editing to Generate Versatile Human Pluripotent Stem Cell Reporter Lines for Analysis of Differentiation and Lineage Tracing

Published:2019-11-13 Issue:12 Volume:37 Page:1556-1566
ISSN:1066-5099
Container-title:Stem Cells
language:en
Short-container-title:

Author:

Bao Xiaoping¹²³,Adil Maroof M.¹²,Muckom Riya¹²,Zimmermann Joshua A.¹²,Tran Aurelie⁴,Suhy Natalie⁴,Xu Yibo³,Sampayo Rocío G.¹²,Clark Douglas S.²⁵,Schaffer David V.¹²³⁴^ORCID

Affiliation:

1. Department of Bioengineering, University of California, Berkeley, California, USA

2. Department of Chemical and Biomolecular Engineering, University of California, Berkeley, California, USA

3. Davidson School of Chemical Engineering, Purdue University, West Lafayette, Indiana, USA

4. Department of Molecular and Cell Biology, University of California, Berkeley, California, USA

5. Department of Chemistry, University of California, Berkeley, California, USA

Abstract

Abstract Transcription factors (TFs) are potent proteins that control gene expression and can thereby drive cell fate decisions. Fluorescent reporters have been broadly knocked into endogenous TF loci to investigate the biological roles of these factors; however, the sensitivity of such analyses in human pluripotent stem cells (hPSCs) is often compromised by low TF expression levels and/or reporter silencing. Complementarily, we report an inducible and quantitative reporter platform based on the Cre-LoxP recombination system that enables robust, quantifiable, and continuous monitoring of live hPSCs and their progeny to investigate the roles of TFs during human development and disease. Stem Cells 2019;37:1556–1566

Publisher

Oxford University Press (OUP)

Subject

Cell Biology,Developmental Biology,Molecular Medicine

Link

https://onlinelibrary.wiley.com/doi/pdf/10.1002/stem.3096

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