Affiliation:
1. Department of Histology and Embryology, Faculty of Veterinary Medicine Burdur Mehmet Akif Ersoy University Burdur Turkey
2. Department of Physiology, Faculty of Veterinary Medicine Kafkas University Kars Turkey
3. Department of Anatomy, Faculty of Veterinary Medicine Atatürk University Erzurum Turkey
4. Department of Biochemistry, Faculty of Veterinary Medicine Atatürk University Erzurum Turkey
5. Department of Bioengineering, Faculty of Engineering and Architecture Kafkas University Kars Turkey
Abstract
ABSTRACTToll‐like receptor 4 (TLR‐4) ligands were initially shown to be the source of lipopolysaccharide (LPS), a gram‐negative bacterium's cell wall immunostimulatory component. Oxidative stress, apoptosis, and inflammation are all potential effects of LPS treatment on the lungs. By triggering oxidative stress and inflammation, these negative effects could be avoided. Robust flavonoid oleuropein (OLE) exhibits anti‐inflammatory, antiproliferative, and antioxidative properties. A nanodelivery system could improve its low bioavailability, making it more effective and useful in treating chronic human ailments. This study evaluates the effects of AgNP‐loaded OLE on LPS‐induced lung injury in rats in terms of TLR4/P2X7 receptor‐mediated inflammation and apoptosis. Forty‐eight male albino rats were randomly divided into eight groups. Drugs were administered to the groups in the doses specified as follows: Control, LPS (8 mg/kg ip), OLE (50 mg/kg) AgNPs (100 mg/kg), OLE + AgNPs (50 mg/kg), LPS + OLE (oleuropein 50 mg/kg ig + LPS 8 mg/kg ip), LPS + AgNPs (AgNPs 100 mg/kg ig + LPS 8 mg/kg ip), and LPS + OLE + AgNPs (OLE + AgNPs 50 mg/kg + LPS 8 mg/kg ip). After the applications, the rats were decapitated under appropriate conditions, and lung tissues were obtained. Oxidative stress (SOD, MDA, and GSH), and inflammation (IL‐6, IL‐1β, TNF‐α, Nrf2, P2X7R, AKT, and TLR4) parameters were evaluated in the obtained lung tissues. Additionally, histopathology studies were performed on lung tissue samples. The data obtained were evaluated by comparison between groups. Both OLE and OLE + AgNPs showed potential in reducing oxidative stress, inflammation, and apoptosis (p < 0.05). These findings were supported by histopathological analysis, which revealed that tissue damage was reduced in OLE and OLE + AgNPs‐treated groups. According to the results, LPS‐induced lung injury can be reduced by using nanotechnology and producing OLE + AgNP.