Affiliation:
1. Hainan Key Laboratory for Sustainable Utilization of Tropical Bioresources, College of Tropical Crops Hainan University Haikou Hainan 570228 China
2. Environment and Plant Protection Institute Chinese Academy of Tropical Agricultural Sciences Haikou Hainan 571737 China
Abstract
AbstractIntroductionFlavonoids are active substances in many herbal medicines, and Areca catechu fruit (AF), an important component in traditional Chinese medicine (TCM), is rich in flavonoids. Different parts of AF, Pericarpium Arecae (PA) and Semen Arecae (SA), have different medicinal effects in prescription of TCM.ObjectiveTo understand flavonoid biosynthesis and regulation in AF.MethodologyThe metabolomic based on liquid chromatography–tandem mass spectrometry (LC–MS/MS) and the transcriptome based on high‐throughput sequencing technology were combined to comprehensively analyse PA and SA.ResultsFrom the metabolite dataset, we found that 148 flavonoids showed significant differences between PA and SA. From the transcriptomic dataset, we identified 30 genes related to the flavonoid biosynthesis pathway which were differentially expressed genes in PA and SA. The genes encoding the key enzymes in the flavonoid biosynthesis pathway, chalcone synthase and chalcone isomerase (AcCHS4/6/7 and AcCHI1/2/3), were significantly higher expressed in SA than in PA, reflecting the high flavonoid concentration in SA.ConclusionsTaken together, our research acquired the key genes, including AcCHS4/6/7 and AcCHI1/2/3, which regulated the accumulation of flavonol in AF. This new evidence may reveal different medicinal effects of PA and SA. This study lays a foundation for investigating the biosynthesis and regulation of flavonoid biosynthesis in areca and provides the reference for the production and consumption of betel nut.
Funder
National Key Research and Development Program of China
National Natural Science Foundation of China
Subject
Complementary and alternative medicine,Drug Discovery,Plant Science,Molecular Medicine,General Medicine,Biochemistry,Food Science,Analytical Chemistry
Cited by
2 articles.
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