The First International eDNA Workshop in Hong Kong: A beginner's guide for the next‐generation eDNA researcher

Author:

Seymour Mathew1ORCID,Guibert Isis1ORCID,Jeunen Gert‐Jan2ORCID,Hellström Micaela3ORCID,Leray Matthieu4ORCID,Elbrect Vasco5,Lau Anthony6,Cutracci Maxine1,How Chun Ming7ORCID,Ip Jack Chi Ho8ORCID,Liu Zifang9,Moffitt Jessica2ORCID,Qian Cheng1,Qin Xian7ORCID,Tse Cham Man1,Wang Yifu1,Wang Zhi10,Zeng Xiaoyi11,Zhao Meihong8

Affiliation:

1. School of Biological Sciences The University of Hong Kong Hong Kong SAR China

2. Department of Anatomy, School of Biomedical Sciences University of Otago Dunedin New Zealand

3. MIX Research Sweden AB Uppsala Sweden

4. Smithsonian Tropical Research Institute Balboa Ancon Panama

5. Independent Researcher and Consultant Zürich Switzerland

6. Lingnan University Hong Kong SAR China

7. City University of Hong Kong Kowloon Hong Kong China

8. Hong Kong Baptist University Hong Kong SAR China

9. Guangdong University of Technology Guangzhou China

10. State Key Laboratory of Marine Environmental Science, College of Ocean and Earth Sciences Xiamen University Xiamen China

11. The Chinese University of Hong Kong Shenzhen China

Abstract

AbstractThe field of environmental DNA (eDNA) has rapidly advanced in recent years, providing a non‐invasive and time‐saving method for assessing biodiversity. The First International Environmental DNA (eDNA) Workshop in Hong Kong was held from 16 to 27 October 2023 and provided early career professionals with hands‐on training and collaboration opportunities in eDNA research. With support from The Croucher Foundation Limited (Hong Kong), the workshop covered all stages of an eDNA‐based research project, including study design, field sampling, eDNA extraction, library preparation, bioinformatics, statistical data analysis, and ethics in scientific research. Participants gained insights into the principles and practical applications of eDNA technology, emphasizing the importance of careful experimental design and data interpretation. The workshop also highlighted the need for standardized protocols, comprehensive and local DNA reference databases, and careful selection of primer sets to overcome current issues and limitations. Workshop participants expressed enthusiasm for the potential of eDNA metabarcoding as a valuable tool for ecological monitoring, biodiversity assessment, and conservation decision‐making. The future of eDNA research looks promising overall, with continued advancements in technology, collaboration among researchers, and the integration of eDNA into large‐scale ecological monitoring. Future iterations of the Hong Kong International eDNA workshop will continue to provide hands‐on training and collaboration opportunities for early career professionals interested in eDNA research, focusing on addressing current limitations and challenges in the field.

Publisher

Wiley

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