A fast and efficient liquid chromatography–tandem mass spectrometry method for measuring l‐ and d‐amino acids in the urine of patients with immunoglobulin A nephropathy

Author:

Zha Zhijian1ORCID,Wang Ruihua1,Wang Qian2,Chen Fahui1,Ye Ziyang1,Li Yafeng13456

Affiliation:

1. Third Clinical School Shanxi University of Traditional Chinese Medicine Taiyuan City Shanxi Province China

2. Shanxi Provincial Key Laboratory of Kidney Disease Taiyuan China

3. Chronic Kidney Disease Medical and Pharmaceutical Basic Research Innovation Center of the Ministry of Education of the People's Republic of China Taiyuan China

4. Department of Nephrology, Shanxi Provincial People's Hospital (Fifth Hospital) Shanxi Medical University Taiyuan China

5. Core Laboratory, Shanxi Provincial People's Hospital (Fifth Hospital) Shanxi Medical University Taiyuan China

6. Academy of Microbial Ecology Shanxi Medical University Taiyuan China

Abstract

AbstractImmunoglobulin nephropathy (IgAN) stands as the most prevalent primary glomerular nephropathy globally, typically diagnosed through an invasive renal biopsy. Emerging research suggests the significant involvement of chiral amino acids in kidney disease progression. This study introduces a nonderivative LC–tandem mass spectrometry approach, offering efficient separation outcomes within 15 min for identifying chiral amino acids in human urine samples. Subsequently, using this method, the analysis of l‐ and d‐amino acids in the urine of both patients with IgAN and healthy individuals was conducted. Fourteen d‐amino acids and 20 l‐amino acids were identified in the urine samples obtained from 17 patients with IgAN and 21 healthy individuals. The results indicated notable variances in the concentrations of both l‐ and d‐amino acids between the IgAN and healthy control groups. In contrast to the healthy group, the IgAN group exhibited higher mean urine concentrations of most l‐amino acids and lower concentrations of d‐amino acids. Furthermore, correlations between amino acids and clinical markers were investigated. These results propose a novel method for monitoring trace amino acids in urine samples and introduce a new concept for potential markers of IgAN.

Funder

National Natural Science Foundation of China

Publisher

Wiley

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