A bicistronic vector with destabilized mRNA secondary structure yields scalable higher titer expression of human neurturin in E. coli

Author:

Roy Varnika1ORCID,Roth Robert2,Berge Mark1,Chitta Rajesh1,Vajrala Sucheta1,Kuntumalla Srilatha3,E. Schmelzer Albert1,Schoner Ron1

Affiliation:

1. Cell Culture and Fermentation Sciences; MedImmune, LLC, 1 MedImmune Way Gaithersburg Maryland 20878

2. Innovative Medicines; AstraZeneca R&D; Mölndal Sweden

3. Analytical Biotechnology; MedImmune, LLC; Gaithersburg Maryland

Publisher

Wiley

Subject

Applied Microbiology and Biotechnology,Bioengineering,Biotechnology

Reference44 articles.

1. The GDNF family: Signalling, biological functions and therapeutic value;Airaksinen;Nat Rev Neurosci,2002

2. Single ribosome dynamics and the mechanism of translation;Aitken;Annu Rev Biophys,2010

3. Multifactorial determinants of protein expression in prokaryotic open reading frames;Allert;J Mol Biol,2010

4. Austen M Burk U. 2005 https://www.google.com/patents/WO2005051415A1

5. GenBank;Benson;Nucleic Acids Res,2013

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