Affiliation:
1. Department of Biology, Texas A&M University , College Station, Texas, USA
Abstract
Abstract
Some extracellular glycoconjugates have sialic acid as the terminal sugar, and sialidases are enzymes that remove this sugar. Mammals have 4 sialidases and can be elevated in inflammation and fibrosis. In this report, we show that incubation of human neutrophils with the extracellular human sialidase NEU3, but not NEU1, NEU2 or NEU4, induces human male and female neutrophils to change from a round to a more amoeboid morphology, causes the primed human neutrophil markers CD11b, CD18, and CD66a to localize to the cell cortex, and decreases the localization of the unprimed human neutrophil markers CD43 and CD62-L at the cell cortex. NEU3, but not the other 3 sialidases, also causes human male and female neutrophils to increase their F-actin content. Human neutrophils treated with NEU3 show a decrease in cortical levels of Sambucus nigra lectin staining and an increase in cortical levels of peanut agglutinin staining, indicating a NEU3-induced desialylation. The inhibition of NEU3 by the NEU3 inhibitor 2-acetylpyridine attenuated the NEU3 effect on neutrophil morphology, indicating that the effect of NEU3 is dependent on its enzymatic activity. Together, these results indicate that NEU3 can prime human male and female neutrophils, and that NEU3 is a potential regulator of inflammation.
Publisher
Oxford University Press (OUP)
Subject
Cell Biology,Immunology,Immunology and Allergy
Reference134 articles.
1. Host immune response to influenza a virus infection;Chen;Front Immunol,2018
2. Recognition of microorganisms and activation of the immune response;Medzhitov;Nature,2007
3. Inflammatory and immune pathways in the pathogenesis of periodontal disease;Cekici;Periodontol 2000,2014
4. Inflammatory responses and inflammation-associated diseases in organs;Chen;Oncotarget,2018
5. Angiogenesis in the pathogenesis of inflammatory joint and lung diseases;Walsh;Arthritis Res,2001
Cited by
2 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献