Association Between Myocardial Oxygenation and Fibrosis in Duchenne Muscular Dystrophy: Analysis by Rest Oxygenation‐Sensitive Magnetic Resonance Imaging

Abstract

BackgroundMyocardial hypoxia has been demonstrated in many cardiomyopathies and is related to development of myocardial fibrosis. However, myocardial hypoxia and its association with myocardial fibrosis are understudied in Duchenne muscular dystrophy (DMD)‐associated cardiomyopathy.PurposeTo evaluate myocardial hypoxia by oxygenation‐sensitive (OS) cardiac magnetic resonance imaging, and further explore its association with fibrosis.Study TypeProspective.SubjectsNinety‐one DMD boys (8.78 ± 2.32) and 30 healthy boys (9.07 ± 2.30).Field Strength/Sequence3 T, Balanced steady‐state free procession, Modified Look‐Locker inversion recovery sequence and Single‐shot phase‐sensitive inversion recovery sequence.AssessmentCardiac MRI data, including left ventricular functional, segmental native T1, and oxygenation signal‐intensity (SI) according to AHA 17‐segment model, were acquired. Patients were divided into LGE+ and LGE− groups. In patients with LGE, all segments were further classified as positive or negative segments by segmentally presence/absence of LGE.Statistical TestsVariables were compared using Student's t, Wilcoxon, Kruskal–Wallis test and one‐way analysis of variance. Bivariate Pearson or Spearman correlation were calculated to determine association between oxygenation SI and native T1. Variables with P < 0.10 in the univariable analysis were included in multivariable model. Receiver operating characteristic analysis was used to assess the performance of OS in diagnosing myocardial hypoxia.ResultsThe myocardial oxygenation SI of DMD was significantly decreased in all segments compared with normal controls, and more obvious in the LGE+ segments (0.46 ± 0.03 vs. 0.52 ± 0.03). For patients with and without LGE, myocardial oxygenation SI were significantly negatively correlated with native T1 in all segments (r = −0.23 to −0.42). The inferolateral oxygenation SI was a significant independent associator of LGE presence (adjusted OR = 0.900).Data ConclusionMyocardial hypoxia evaluated by the OS‐Cardiac‐MRI indeed occurs in DMD and associate with myocardial fibrosis, which might be used as a biomarker in assessing myocardial damage in DMD.Evidence Level1Technical EfficacyStage 1