Affiliation:
1. Department of obstetrics Affiliated Hospital of Putian University Putian University Putian 351100 China
2. Central laboratory Affiliated Hospital of Putian University Putian University Putian 351100 China
3. Department of rehabilitation medicine Affiliated Hospital of Putian University Putian University Putian 351100 China
4. Ministry of Education Key Laboratory for Analytical Science of Food Safety and Biology Department of Chemistry Fuzhou University Fuzhou 350116 China
Abstract
AbstractOxidative stress caused by pregnancy‐induced hypertension syndrome significantly affects the health of pregnant women. Hydrogen sulfide is a typical gaseous signal molecule against oxidative stress, and it is of profound significance to develop a detection method. In this study, a stimuli‐responsive hydrogel was constructed based on the coordination and bonding principle of metal ions and chitosan (CS) to realize the quantitative detection of hydrogen sulfide (H2S). The chain of CS contains a large number of amino groups and hydroxyl groups, which can form the coordination structure with Cu2+, triggering CS to form a stable hydrogel. The hydrogel can be formed within about 5 s, which has the characteristics of rapid preparation. In the presence of target H2S, the cross‐linking agent Cu2+ in the hydrogel can compete out, resulting in the collapse of the hydrogel and the release of the electrochemical probe. By detecting the concentration of the released electrochemical probe, the quantitative detection of H2S can be achieved. The prepared hydrogel has a good linear relationship with the concentration of H2S from 1 μM to 60 μm. At the same time, the hydrogel has good specificity and stability, and it can be applied to the detection of H2S in serum samples.
Funder
National Natural Science Foundation of China
Natural Science Foundation of Fujian Province