Methylopila

Abstract

Abstract Me.thy.lo.pi'la. N.L. neut. n. methyl , the methyl group; L. fem. n. pila , ball or sphere; N.L. Methylopila , methyl‐using sphere. Pseudomonadota / Alphaproteobacteria / Rhizobiales / Methylopilaceae / Methylopila The genus Methylopila accommodates strictly aerobic facultatively methylotrophic bacteria. Cells are Gram‐stain‐negative, rod shaped, asporogenous, and motile or nonmotile; occur singly or in pairs; multiply by binary fission; and contain poly‐β‐hydroxybutyrate granules. Utilize methanol and methylamine as well as a variety of multicarbon compounds as carbon and energy sources. Assimilate C 1 compounds by isocitrate lyase‐negative variant of the serine pathway. Growth factors are not required. Ammonium salts, nitrate, urea, peptone, some amino acids, and methylated amines are utilized as nitrogen sources. Do not grow on CH 4 or H 2 /CO 2 /O 2 . Oxidase‐, catalase‐, and urease‐positive. Indole is formed from l ‐tryptophan on mineral medium with methanol as the sole carbon and energy source and KNO 3 as the sole nitrogen source. Acids are produced from sugars oxidatively. Growth occurs at 10–37°C (but not at 42°C), pH 5.0–10.0. The major fatty acid is C 18:1 ω7 c . The major quinone is Q‐10. The genome sizes are 3.72–4.14 Mb. According to Genome Taxonomy Database (GTDB), the genus belongs to the phylum Pseudomonadota , class Alphaproteobacteria , order Rhizobiales , and family Methylopilaceae . Representatives of this genus inhabit phyllosphere and rhizosphere of various plants, fruits, and activated sludge, polluted and natural soils. DNA G + C content (mol%) : 64.5–70.4 ( T m and genome analyses). Type species : Methylopila capsulata Doronina et al. 1998 VP .