Development and optimization of Lysis gene <i>E</i> as a counter‐selection marker with high stringency-Reference-Cited by-同舟云学术

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Development and optimization of Lysis gene E as a counter‐selection marker with high stringency

Published:2022-04-17 Issue:8 Volume:17 Page:2100423
ISSN:1860-6768
Container-title:Biotechnology Journal
language:en
Short-container-title:Biotechnology Journal

Author:

Chen Wei¹²^ORCID,Chen Ruyi¹,He Ling²,Wu Xiaotong¹

Affiliation:

1. Guangdong Province Key Laboratory for Biotechnology Drug Candidates School of Biosciences and Biopharmaceutics Guangdong Pharmaceutical University Guangzhou China

2. Guangdong Key Laboratory of Pharmaceutical Bioactive Substances Guangdong Pharmaceutical University Guangzhou China

Publisher

Wiley

Subject

Molecular Medicine,Applied Microbiology and Biotechnology,General Medicine

Link

https://onlinelibrary.wiley.com/doi/pdf/10.1002/biot.202100423

Reference39 articles.

1. Integration of DNA into bacterial chromosomes from plasmids without a counter-selection marker

2. Using the Cre/lox system for targeted integration into the human genome: loxFAS-loxP pairing and delayed introduction of Cre DNA improve gene swapping efficiency

3. An Fgf8 mutant allelic series generated by Cre- and Flp-mediated recombination

4. A Highly Efficient Escherichia coli-Based Chromosome Engineering System Adapted for Recombinogenic Targeting and Subcloning of BAC DNA

5. Modification of human beta-globin locus PAC clones by homologous recombination in Escherichia coli

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