MCM2 is involved in subtyping and tamoxifen resistance of ERα‐positive breast cancer by acting as the downstream factor of ERα

Abstract

AbstractTamoxifen (TAM) resistance is finally developed in over 40% of patients with estrogen receptor α‐positive breast cancer (ERα+‐BC), documenting that discovering new molecular subtype is needed to confer perception to the heterogeneity of ERα+‐BC. We obtained representative gene sets subtyping ERα+‐BC using gene set variation analysis (GSVA), non‐negative matrix factorization (NMF), and COX regression methods on the basis of METABRIC, TCGA, and GEO databases. Furthermore, the risk score of ERα+‐BC subtyping was established using least absolute shrinkage and selection operator (LASSO) regression on the basis of genes in the representative gene sets, thereby generating the two subtypes of ERα+‐BC. We further found that minichromosome maintenance complex component 2 (MCM2) functioned as the hub gene subtyping ERα+‐BC using GO, KEGG, and MCODE. MCM2 expression was capable for specifically predicting 1‐year overall survival (OS) of ERα+‐BC and correlated with T stage, AJCC stage, and tamoxifen (TAM) sensitivity of ERα+‐BC. The downregulation of MCM2 expression inhibited proliferation, migration, and invasion of TAM‐resistant cells and promoted G0/G1 arrest. Altogether, tamoxifen resistance entails that MCM2 is a hub gene subtyping ERα+‐BC, providing a novel dimension for discovering a potential target of TAM‐resistant BC.