In vitro and in vivo comparative analysis of antibacterial activity of green‐synthesized silver nanoparticles

Author:

Lethongkam Sakkarin123,Glaser Jochen4,Ammanath Aparna Viswanathan2,Voravuthikunchai Supayang P.13ORCID,Götz Friedrich2

Affiliation:

1. Division of Biological Science Faculty of Science Prince of Songkla University Hat Yai Songkhla Thailand

2. Microbial Genetics Institute of Microbiology and Infection Medicine Tübingen University of Tübingen Tübingen Germany

3. Center of Antimicrobial Biomaterial Innovation‐Southeast Asia Prince of Songkla University Hat Yai Songkhla Thailand

4. Institut für Anorganische Chemie University of Tübingen Tübingen Germany

Abstract

AbstractThis study aims to compare antibacterial effects of green‐synthesized silver nanoparticles (AgNPs) with silver nitrate (AgNO3). AgNPs were successfully synthesized using Eucalyptus camaldulensis leaf extract as a reducing and stabilizing agent. Minimum inhibitory concentrations (MIC) of AgNPs and AgNO3 against Staphylococcus aureus and Pseudomonas aeruginosa ranged between 4.8 and 6.75 µg mL−1. Growth curves demonstrated that inhibition of P. aeruginosa occurred right after AgNPs were added and throughout the period of the study (72 h). Antibacterial effects of both AgNPs and AgNO3 could be abrogated by cysteine and 2‐mercaptoethanol, thiol‐containing compounds. Galleria mellonella model revealed relatively low toxic effects of both AgNPs and AgNO3. At 20MIC of AgNPs (≈137.8 mg kg−1), more than 80% survival of G. mellonella was observed. Unexpectedly, silver‐containing agents could not rescue larvae after S. aureus infection. Further ex vivo experiments in the presence of coelomic larval fluid demonstrated the reduction of antibacterial activity of both AgNPs and AgNO3. It was speculated that anionic molecules present in the coelomic fluid might neutralize the action of Ag ions. Binding of AgNPs or AgNO3 to albumin, a major protein in human blood which transport several endogenous compounds was not detected, indicating that the silver‐containing agents could be applied as an antimicrobial agent.

Funder

National Research Council of Thailand

Deutsche Forschungsgemeinschaft

Publisher

Wiley

Subject

Molecular Medicine,Applied Microbiology and Biotechnology,General Medicine

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