Exosomal lncRNA USP30‐AS1 activates the Wnt/β‐catenin signaling pathway to promote cervical cancer progression via stabilization of β‐catenin by USP30

Author:

Chi Chi1,Tang Xiuwu1,Liu Wei1,Zhou Ying1,Jiang Rong1,Chen Youguo1,Li Min1ORCID

Affiliation:

1. Department of Obstetrics and Gynecology The First Affiliated Hospital of Soochow University Suzhou Jiangsu China

Abstract

AbstractCervical cancer (CC) remains a major cause of cancer‐related mortality among women globally. Long noncoding RNAs (lncRNAs) play crucial regulatory roles in various cancers, including CC. This study investigates the function of a novel lncRNA, USP30 antisense RNA 1 (USP30‐AS1), in CC tumorigenesis. We analyzed USP30‐AS1 expression using RT‐qPCR and conducted in vitro loss‐of‐function assays, as well as in vivo assays, to evaluate the effects of USP30‐AS1 silencing on CC cell growth and migration. Additional mechanistic experiments, including RNA pull‐down, RNA immunoprecipitation (RIP), and co‐immunoprecipitation (Co‐IP) assays, were performed to elucidate the regulatory mechanisms influenced by USP30‐AS1. We discovered that USP30‐AS1 is overexpressed in CC tissues and cells. Silencing USP30‐AS1 significantly reduced cell proliferation, migration, invasion, and tumor growth. Moreover, USP30‐AS1 was found to modulate the expression of ubiquitin‐specific peptidase 30 (USP30) by sponging microRNA‐2467‐3p (miR‐2467‐3p) and recruiting the FUS RNA binding protein (FUS), thereby stabilizing β‐catenin and activating the Wnt/β‐catenin signaling pathway. These findings suggest that USP30‐AS1 enhances CC cell growth and migration through the miR‐2467‐3p/FUS/USP30 axis, highlighting its potential as a biomarker for CC.

Funder

National Natural Science Foundation of China

Publisher

Wiley

同舟云学术

1.学者识别学者识别

2.学术分析学术分析

3.人才评估人才评估

"同舟云学术"是以全球学者为主线,采集、加工和组织学术论文而形成的新型学术文献查询和分析系统,可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容,当前同舟云学术共收录了国内外主流学术期刊6万余种,收集的期刊论文及会议论文总量共计约1.5亿篇,并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询!咨询电话:010-8811{复制后删除}0370

www.globalauthorid.com

TOP

Copyright © 2019-2024 北京同舟云网络信息技术有限公司
京公网安备11010802033243号  京ICP备18003416号-3