Development of disposable electrode for the detection of mosquito‐borne viruses

Abstract

AbstractDevelopment of disposable, rapid, and convenient biosensor with high sensitivity and reliability is the most desired method of viral disease prevention. To achieve this goal, in this work, a practical impedimetric biosensor has been implemented into a disposable electrode on a screen‐printed carbon electrode (SPCE) for the detection of two mosquito‐borne viruses. The biosensor fabrication has step‐wisely carried out on the disposable electrode surface at room temperature: starting from conductive film formation, physical binding of the gold nanoparticles (AuNPs)‐polyaniline (PAni) into the conductive film, and biofunctionalization. To get the maximum efficiency of the antibody, biotinylated antibody has been conjugated on the surface of AuNP‐PAni/PAni‐SPCE via the streptavidin‐biotin conjugation method which is a critical factor for the high sensitivity. Using the antibody–antigen interaction, this disposable electrode has designed to detect mosquito‐borne infectious viruses, Chikungunya virus (CHIKV), and Zika virus (ZIKV) separately in a wide linear range of 100 fg mL−1 to 1 ng mL−1 with a low detection limit of 1.33 and 12.31 fg mL−1, respectively.