Linoleic acid derivatives target miR‐361‐3p/BTG2 to confer anticancer effects in acute myeloid leukemia

Abstract

AbstractAcute myeloid leukemia (AML) is a deadly hematologic malignancy. In this study, miR‐361‐3p and BTG2 gene expression in AML blood and healthy specimens were analyzed using quantitative real‐time reverse transcription polymerase chain reaction. A significant negative correlation between miR‐361‐3p and BTG2 was observed. The cell viability and apoptosis were measured by CCK‐8 assay, EdU incorporation assay and flow cytometry. A dual‐luciferase reporter gene assay was performed to confirm the binding sequence between miR‐361‐3p and BTG2 messenger RNA 3ʹ‐untranslated region. 9s‐Hydroxyoctadecadienoic acid (9s‐HODE), a major active derivative of linoleic acid, reduced the viability and induced cell apoptosis of HL‐60 cells. Furthermore, the miR‐361‐3p mimics and siBTG2 reversed the above effects of 9s‐HODE. 9s‐HODE exerted an anti‐AML effect through, at least partly, regulating the miR‐361‐3p/BTG2 axis.