Impact of nucleic acid extraction procedures on human papillomavirus (HPV) detection and genotyping

Abstract

AbstractHuman papillomavirus (HPV) infections are often asymptomatic, but some of the >200 HPV genotypes confer a high risk for precancerous cervical lesions and cervical cancer. Current clinical management of HPV infections relies on reliable nucleic acid testing detection and genotyping. We prospectively compared nucleic acid extraction without and with prior centrifugation enrichment for detecting and genotyping HPV in cervical swabs with atypical squamous or glandular cells. Consecutive swabs were analyzed from 45 patients with atypical squamous or glandular cells. Nucleic acids were extracted in parallel using three procedures, Abbott‐M2000, Roche‐MagNA‐Pure‐96 Large‐Volume Kit without (Roche‐MP‐large) and with prior centrifugation (Roche‐MP‐large/spin) and tested using Seegene‐Anyplex‐II HPV28. In total, 54 HPV‐genotypes were detected in 45 samples, 51 by Roche‐MP‐large/spin, 48 by Abbott‐M2000 and 42 by Roche‐MP‐large. The overall concordance was 80% for detecting any HPV and 74% for specific HPV‐genotypes. Roche‐MP‐large/spin and Abbott‐M2000 showed the highest concordance for HPV detection (88.9%; kappa 0.78), and genotyping (88.5%). Two and more HPV‐genotypes were detected in 15 samples, often with one HPV being more abundant. Dilution series confirmed the specific detection of multiple HPV‐genotypes and their relative abundance. In 285 consecutive follow‐up samples extracted by Roche‐MP‐large/spin, the top three detected genotypes were the high‐risk HPV16, HPV53, HPV56 and the low‐risk HPV42, HPV54 and HPV61. Rate and breadth of HPV detection in cervical swabs depends on extraction protocols being highest after centrifugation/enrichment. As multivalent HPV‐vaccine coverage increases, detecting the evolving HPV virome depends on improved extraction and broader genotype coverage.