Affiliation:
1. Department of Internal Medicine Yuyao Traditional Chinese Medicine Hospital Yuyao Zhejiang China
2. Department of Encephalopathy Chongqing Traditional Chinese Medicine Hospital Chongqing China
Abstract
AbstractBackgroundThis study attempts to investigate the therapeutic effect of sinomenine on renal fibrosis and its mechanism.MethodsThe 8‐week‐old C57BL/6 male mice were randomly divided into sham group, UUO model group, UUO sinomenine group (UUO + Sino 50), UUO + sinomenine group (UUO + Sino 100), UUO + exosome group (exo), and UUO + exo‐inhibitor. The pathological changes of kidney were observed by H&E staining, the degree of renal interstitial fibrosis was detected by MASSON and Sirius red staining, and the expressions of fibrosis and autophagy markers were detected by real‐time fluorescence quantitative PCR and WB. NTA and electron microscopy were used to analyze exo secretion after sinomenine treatment.ResultsSinomenine could improve the progression of renal fibrosis without causing tissue damage including heart, lungs and liver. Sinomenine could promote autophagosome formation. It could promote the secretion of exosomes from bone marrow mesenchymal stem cells (BMSCs). Sinomine regulates the PI3K‐AKT pathway through BMSC‐exo carrying miR‐204‐5p, affecting autophagy level and alleviating the process of renal fibrosis.ConclusionOur study suggests that sinomine could improve the progression of renal fibrosis by influencing the expression of miR‐204‐5p in BMSC‐exo and regulating the PI3K‐AKT pathway.
Subject
Health, Toxicology and Mutagenesis,Management, Monitoring, Policy and Law,Toxicology,General Medicine
Cited by
1 articles.
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