Affiliation:
1. Department of Ultrasound Xinqiao Hospital, Army Medical University Chongqing China
2. Department of Ultrasound Postgraduate Training Basement of Jinzhou Medical University, The PLA Rocket Force Characteristic Medical Center Beijing China
3. Department of Ultrasound 953th Hospital, Shigatse Branch, Xinqiao Hospital, Army Medical University Shigatse China
Abstract
ObjectivesUltrasound‐targeted microbubble disruption (UTMD) is a widely used technique to improve the differentiation and proliferation capacity of mesenchymal stem cells (MSCs), but the optimal therapeutic parameters for UTMD are unclear. In this study, we aimed to find the appropriate peak negative pressure (PNP), which is a key parameter for enhancing the stemness properties and proliferation of MSCs.MethodsExperiments were performed in UTMD group, ultrasound (US) group under different PNP exposure conditions (0.5, 1.0, and 1.5 MPa), and control group. Apoptosis safety was analyzed by flow cytometry and MSC proliferation was measured at 12, 24, and 36 hours after irradiation by cell counting kit 8. The expression of the stemness genes NANOG, OCT‐4, and SOX‐2 were determined by enzyme‐linked immunosorbent assay (ELISA) or reverse transcription polymerase chain reaction.ResultsThe results showed that the 1.5 MPa UTMD‐treated group had the highest proliferation capacity of MSCs at 24 hours. ELISA or quantitative reverse transcription polymerase chain reaction results showed that UTMD treatment of the 1.5 MPa group significantly upregulated the expression of the stemness genes NANOG, SOX‐2, and OCT‐4.ConclusionsIn conclusion, the appropriate peak PNP value of UTMD was 1.5 MPa, and 1.5 MPa‐mediated UTMD group obviously promoted MSCs proliferation and maintained stemness by upregulating the expression of stemness genes.