Affiliation:
1. College of Polymer Science and Engineering State Key Laboratory of Polymer Materials Engineering Department of Ultrasound West China Hospital Sichuan University Chengdu 610065 China
2. Med‐X Center for Materials Sichuan University Chengdu 610041 China
3. State Key Laboratory of Oral Diseases National Clinical Research Center for Oral Diseases West China Hospital of Stomatology Sichuan University Chengdu 610041 China
Abstract
AbstractExploring rapid, sensitive, and multifunctional biosensors is of great significance in medical diagnosis and biotechnology, while creating Cu‐N‐C center‐based enzymatic biodetection system is still a great challenge for colorimetric biosensing fields. Herein, inspired by the axial coordination structure of the natural enzyme, an efficient and specific Cu‐N‐C center‐based peroxidase‐like biocatalyst is created via axial strong‐metal‐support‐interaction (SMSI) between WOx support and Cu‐Nx‐based carbon shell, denoted as Cu‐NC‐WO3. This analysis discloses that the SMSI effect induces electron transfer from Cu to WOx, thus making the valence state of Cu higher and consequently suppressing the O intermediates' adsorption. Owing to this unique structure, the Cu‐NC‐WO3 exhibits exceptional peroxidase (POD) like activities (Km = 3.57 × 10−3 m, Vmax = 13.80 × 10−6 m s−1, and turnover number (TON) = 206.13 × 10−3 s−1 for H2O2), which is more efficient than the other Cu‐Nx center‐based biocatalysts and also many recently reported peroxidase‐like nanomaterials. Meanwhile, this Cu‐NC‐WO3 shows substrate selectivity, low detection limit, and high resistance to extreme environments for abundant H2O2‐related biomarkers, which are revealed by colorimetric studies. Thus, as a helpful method for accurate biodetection, it is believed this work promises to offer rapid, specific, and inexpensive colorimetric biosensors for regions with bare medical resources, as well as new strategies for the structural engineering of enzyme‐like biocatalysts.
Funder
State Key Laboratory of Polymer Materials Engineering
Subject
Industrial and Manufacturing Engineering,Mechanics of Materials,General Materials Science
Cited by
1 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献