Benchmarking sample preparation/digestion protocols reveals tube-gel being a fast and repeatable method for quantitative proteomics
Author:
Affiliation:
1. Laboratoire de Spectrométrie de Masse BioOrganique; Université de Strasbourg; CNRS, IPHC UMR 7178 Strasbourg France
Funder
Agence Nationale de la Recherche
French Proteomic Infrastructure
Publisher
Wiley
Subject
Molecular Biology,Biochemistry
Link
http://onlinelibrary.wiley.com/wol1/doi/10.1002/pmic.201600288/fullpdf
Reference21 articles.
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2. Simple sodium dodecyl sulfate-assisted sample preparation method for LC-MS-based proteomics applications;Zhou;Anal. Chem.,2012
3. In-gel digestion of proteins for internal sequence analysis after one- or two-dimensional gel electrophoresis;Rosenfeld;Anal. Biochem.,1992
4. Label-free quantification and shotgun analysis of complex proteomes by one-dimensional SDS-PAGE/NanoLC-MS: evaluation for the large scale analysis of inflammatory human endothelial cells;Gautier;Mol. Cell. Proteomics,2012
5. Tube-gel digestion: a novel proteomic approach for high throughput analysis of membrane proteins;Lu;Mol. Cell. Proteomics,2005
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