Affiliation:
1. School of Biotechnology Jiangnan University 1800 Lihu Road Wuxi 214122 China
2. College of Food Science and Technology Nanjing Agricultural University Nanjing Jiangsu 210095 China
3. Departments of Chemical & Biomolecular Engineering and of Bioengineering University of California Berkeley Berkeley CA 94720 USA
4. Biological Systems and Engineering Division Lawrence Berkeley National Laboratory Berkeley CA 94720 USA
5. Center for Biosustainability Technical University of Denmark Lyngby 2800 Denmark
6. Science Center for Future Foods Jiangnan University 1800 Lihu Road Wuxi Jiangsu 214122 China
Abstract
AbstractSelf‐assembling peptides are valuable building blocks to fabricate supramolecular biomaterials, which have broad applications from biomedicine to biotechnology. However, limited choices to induce different globular proteins into hydrogels hinder these designs. Here, an easy‐to‐implement and tunable self‐assembling strategy, which employs Ure2 amyloidogenic peptide, are described to induce any target proteins to assemble into supramolecular hydrogels alone or in combination with notable compositional control. Furthermore, the collective effect of nanoscale interactions among amyloid nanofibrils and partially disordered elastomeric polypeptides are investigated. This led to many useful macroscopic material properties simultaneously emerging from one pure protein material, i.e. strong adhesion to any substrates under wet conditions, rapidly self‐‐assembling into robust and porous hydrogels, adaptation to remodeling processes, strongly promoting cell adhesion, proliferation and differentiation. Moreover, he demonstrated this supramolecular material′s robust performance in vitro and vivo for tissue engineering, cosmetic and hemostasis applications and exhibited superior performance compared to corresponding commercial counterparts. To the best of his knowledge, few pure protein‐based materials could meet such seemingly mutually exclusive properties simultaneously. Such versatility renders this novel supramolecular nanomaterial as next‐generation functional protein‐based materials, and he demonstrated the sequence level modulation of structural order and disorder as an untapped principle to design new proteins.
Funder
Natural Science Foundation of Jiangsu Province
Excellent Youth Foundation of Jiangsu Scientific Committee
Jiangsu Agricultural Science and Technology Innovation Fund
National Natural Science Foundation of China
Fundamental Research Funds for the Central Universities
China Postdoctoral Science Foundation
Subject
Mechanical Engineering,Mechanics of Materials,General Materials Science