Affiliation:
1. Biological and Soft Systems Cavendish Laboratory University of Cambridge JJ Thomson Avenue Cambridge CB3 0HE UK
2. Department of Chemical Engineering and Biotechnology University of Cambridge Philippa Fawcett Drive Cambridge CB3 0AS UK
3. Department of Chemistry Molecular Sciences Research Hub Imperial College London Wood Lane London W12 0BZ UK
4. fabriCELL Molecular Sciences Research Hub Imperial College London Wood Lane London W12 0BZ UK
Abstract
AbstractState‐of‐the‐art bottom‐up synthetic biology allows to replicate many basic biological functions in artificial‐cell‐like devices. To mimic more complex behaviors, however, artificial cells would need to perform many of these functions in a synergistic and coordinated fashion, which remains elusive. Here, a sophisticated biological response is considered, namely the capture and deactivation of pathogens by neutrophil immune cells, through the process of netosis. A consortium consisting of two synthetic agents is designed—responsive DNA‐based particles and antibiotic‐loaded lipid vesicles—whose coordinated action mimics the sought immune‐like response when triggered by bacterial metabolism. The artificial netosis‐like response emerges from a series of interlinked sensing and communication pathways between the live and synthetic agents, and translates into both physical and chemical antimicrobial actions, namely bacteria immobilization and exposure to antibiotics. The results demonstrate how advanced life‐like responses can be prescribed with a relatively small number of synthetic molecular components, and outlines a new strategy for artificial‐cell‐based antimicrobial solutions.
Funder
H2020 European Research Council
UK Research and Innovation
Leverhulme Trust
Subject
Mechanical Engineering,Mechanics of Materials,General Materials Science
Cited by
3 articles.
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