Coacervation‐Mediated Cytocompatible Formation of Supramolecular Hydrogels with Self‐Evolving Macropores for 3D Multicellular Spheroid Culture

Abstract

AbstractCoacervation driven liquid–liquid phase separation of biopolymers has aroused considerable attention for diverse applications, especially for the construction of microstructured polymeric materials. Herein, a coacervate‐to‐hydrogel transition strategy is developed to create macroporous hydrogels (MPH), which are formed via the coacervation process of supramolecular assemblies (SA) built by the host–guest complexation between γ‐cyclodextrin and anthracene dimer. The weak and reversible supramolecular crosslinks endow the SA with liquid‐like rheological properties, which facilitate the formation of SA‐derived macroporous coacervates and the subsequent transition to MPH (pore size ≈ 100 µm). The excellent structural dynamics (derived from SA) and the cytocompatible void‐forming process of MPH can better accommodate the dramatic volumetric expansion associated with colony growth of encapsulated multicellular spheroids compared with the non‐porous static hydrogel with similar initial mechanical properties. The findings of this work not only provide valuable guidance to the design of biomaterials with self‐evolving structures but also present a promising strategy for 3D multicellular spheroid culture and other diverse biomedical applications.