Affiliation:
1. Department of Breast Surgery Caner Hospital of Yunnan Province The Third Affiliated Hospital of Kunming Medical University Kunming China
2. State Key Laboratory of Primate Biomedical Research Institute of Primate Translational Medicine Kunming University of Science and Technology Kunming China
3. Yunnan Key Laboratory of Primate Biomedical Research Kunming China
4. Department of Ultrasound Caner Hospital of Yunnan Province The Third Affiliated Hospital of Kunming Medical University Kunming China
5. Department of Cardiothoracic Surgery Baoshan People's Hospital Baoshan China
6. Center for Genomic and Personalized Medicine Guangxi Medical University Nanning China
7. Department of Immunology School of Basic Medical Sciences Guangxi Medical University Nanning China
Abstract
AbstractBackgroundBreast cancer (BC) is a highly heterogeneous disease, and although immunotherapy has recently increased patient survival in a number of solid and hematologic malignancies, most BC subtypes respond poorly to immune checkpoint blockade therapy (ICB). B cells, particularly those that congregate in tertiary lymphoid structures (TLS), play a significant role in antitumour immunity. However, B‐cell heterogeneity at single‐cell resolution and its clinical significance with TLS in BC need to be explored further.MethodsPrimary tumour lesions and surrounding normal tissues were taken from 14 BC patients, totaling 124,587 cells, for single‐cell transcriptome sequencing and bioinformatics analysis.ResultsBased on the usual markers, the single‐cell transcriptome profiles were classified into various clusters. A thorough single‐cell study was conducted with a focus on tumour‐infiltrating B cells (TIL‐B) and tumour‐associated neutrophils (TAN). TIL‐B was divided into five clusters, and unusual cell types, such as follicular B cells, which are strongly related to immunotherapy efficacy, were identified. In BC, TAN and TIL‐B infiltration are positively correlated, and at the same time, compared with TLS‐high, TAN and TIL‐B in TLS‐low group are significantly positively correlated.ConclusionsIn conclusion, our study highlights the heterogeneity of B cells in BC, explains how B cells and TLS contribute significantly to antitumour immunity at both the single‐cell and clinical level, and offers a straightforward marker for TLS called CD23. These results will offer more pertinent information on the applicability and effectiveness of tumour immunotherapy for BC.
Funder
National Natural Science Foundation of China
Natural Science Foundation of Yunnan Province
Subject
Molecular Medicine,Medicine (miscellaneous)
Cited by
5 articles.
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