Uptake of extracellular vesicles into immune cells is enhanced by the protein corona

Author:

Dietz Laura12,Oberländer Jennifer12,Mateos‐Maroto Ana2ORCID,Schunke Jenny12,Fichter Michael12,Krämer‐Albers Eva‐Maria3ORCID,Landfester Katharina2ORCID,Mailänder Volker12ORCID

Affiliation:

1. Department of Dermatology University Medical Center Mainz Mainz Germany

2. Max Planck Institute for Polymer Research Mainz Germany

3. Institute of Developmental Biology and Neurobiology Johannes Gutenberg University of Mainz Mainz Germany

Abstract

AbstractThe influence of a protein corona on the uptake of nanoparticles in cells has been demonstrated in various publications over the last years. Extracellular vesicles (EVs), can be seen as natural nanoparticles. However, EVs are produced under different cell culture conditions and little is known about the protein corona forming on EVs and its influence on their uptake by target cells. Here, we use a proteomic approach in order to analyze the protein composition of the EVs themselves and the protein composition of a human blood plasma protein corona around EVs. Moreover, we analyze the influence of the protein corona on EV uptake into human monocytes and compare it with the influence on the uptake of engineered liposomes. We show that the presence of a protein corona increases the uptake of EVs in human monocytes. While for liposomes this seems to be triggered by the presence of immunoglobulins in the protein corona, for EVs blocking the Fc receptors on monocytes did not show an influence of uptake. Therefore, other mechanisms of docking to the cell membrane and uptake are most like involved, demonstrating a clear difference between EVs and liposomes as technically produced nanocarriers.

Funder

Deutsche Forschungsgemeinschaft

Publisher

Wiley

Subject

Cell Biology,Histology

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