Affiliation:
1. Division of Cardiology, Department of Internal Medicine Yonsei University College of Medicine Seodaemun‐gu Seoul Republic of Korea
2. Department of Biochemistry and Molecular Biology Yonsei University College of Medicine Seodaemun‐gu Seoul Republic of Korea
3. GNTPharma Science and Technology Center for Health, Giheung‐gu Yongin‐si Incheon Republic of Korea
4. Graduate School of Medical Science, Brain Korea 21 Project Yonsei University College of Medicine Seodaemun‐gu Seoul Republic of Korea
Abstract
AbstractSmall‐interfering RNA (siRNA) therapy is considered a powerful therapeutic strategy for treating cardiac hypertrophy, an important risk factor for subsequent cardiac morbidity and mortality. However, the lack of safe and efficient in vivo delivery of siRNAs is a major challenge for broadening its clinical applications. Small extracellular vesicles (sEVs) are a promising delivery system for siRNAs but have limited cell/tissue‐specific targeting ability. In this study, a new generation of heart‐targeting sEVs (CEVs) has been developed by conjugating cardiac‐targeting peptide (CTP) to human peripheral blood‐derived sEVs (PB‐EVs), using a simple, rapid and scalable method based on bio‐orthogonal copper‐free click chemistry. The experimental results show that CEVs have typical sEVs properties and excellent heart‐targeting ability. Furthermore, to treat cardiac hypertrophy, CEVs are loaded with NADPH Oxidase 4 (NOX4) siRNA (siNOX4). Consequently, CEVs@siNOX4 treatment enhances the in vitro anti‐hypertrophic effects by CEVs with siRNA protection and heart‐targeting ability. In addition, the intravenous injection of CEVs@siNOX4 into angiotensin II (Ang II)‐treated mice significantly improves cardiac function and reduces fibrosis and cardiomyocyte cross‐sectional area, with limited side effects. In conclusion, the utilization of CEVs represents an efficient strategy for heart‐targeted delivery of therapeutic siRNAs and holds great promise for the treatment of cardiac hypertrophy.
Funder
Ministry of Education
Ministry of Science and ICT, South Korea
Cited by
2 articles.
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