Microsporogenesis and the biosynthesis of floral small interfering RNAs in coffee have a unique pattern among eudicots, suggesting a sensitivity to climate changes

Author:

de Oliveira Kellen Kauanne Pimenta1ORCID,de Oliveira Raphael Ricon1ORCID,de Campos Rume Gabriel1,Ribeiro Thales Henrique Cherubino1ORCID,Fernandes‐Brum Christiane Noronha1,do Amaral Laurence Rodrigues2,Kakrana Atul3,Mathioni Sandra3,Meyers Blake C.34ORCID,de Souza Gomes Matheus2,Chalfun‐Junior Antonio1ORCID

Affiliation:

1. Laboratory of Plant Molecular Physiology, Plant Physiology Sector Institute of Biology, Federal University of Lavras (UFLA) Lavras Minas Gerais Brazil

2. Laboratory of Bioinformatics and Molecular Analysis Federal University of Uberlândia (UFU) Campus Patos de Minas Patos de Minas Minas Gerais Brazil

3. Donald Danforth Plant Science Center St. Louis MO USA

4. Division of Plant Science and Technology University of Missouri‐Columbia Columbia Missouri USA

Abstract

AbstractRecently, the siRNAs pathways, and especially reproductive phasiRNAs, have attracted attention in eudicots since their biological roles are still unknown and their biogenesis took different evolutionary pathways compared to monocots. In this work, we used Coffea arabica L., a recent allotetraploid formed from the hybridization of Coffea canephora and C. eugenioides unreduced gametes, to explore microsporogenesis and small RNAs‐related pathways in a eudicot crop. First, we identified the microsporogenesis stages during anther development revealing that pre‐meiosis occurs in anthers of 1.5 mm inside floral buds (FBs), whereas meiosis between 1.5 and 4.2 mm FBs, and post‐meiosis in FBs larger than 4.2 mm. These stages coincide with the Brazilian winter, a period of FBs reduced growth which suggests temperature sensitivity. Next, we identified and quantified the expression of reproductive 21‐ and 24‐nt phasiRNAs during coffee anther development together with their canonical and novel miRNA triggers, and characterized the DCL and AGO families. Our results showed that the pattern of reproductive phasiRNA abundance in C. arabica is unique among described eudicots and the canonical trigger car‐miR2275 is involved in the processing of both 21‐ and 24‐nt phasiRNAs. Fourteen DCL genes were identified, but DCL5, related to phasiRNA biosynthesis in monocots, was not, according to its specificity for monocots. Thus, our work explored the knowledge gap about microsporogenesis and related siRNAs pathways in coffee, contributing to the control of reproductive development and the improvement of fertility in eudicots.

Publisher

Wiley

Subject

Plant Science,Biochemistry, Genetics and Molecular Biology (miscellaneous),Ecology,Ecology, Evolution, Behavior and Systematics

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