Is there section deformation resulting in differential change of nuclear numerical densities along the z axis of thick methacrylate or paraffin sections?
Author:
Affiliation:
1. Experimental Teaching Center of Functional ScienceNorth Sichuan Medical College Nanchong Sichuan China
2. Morphometric Research LaboratoryNorth Sichuan Medical College Nanchong Sichuan China
Publisher
Wiley
Subject
Medical Laboratory Technology,Instrumentation,Histology,Anatomy
Link
https://onlinelibrary.wiley.com/doi/pdf/10.1002/jemt.23323
Reference23 articles.
1. Pronounced loss of cell nuclei and anisotropic deformation of thick sections
2. Estimation of surface area from vertical sections
3. Two distinct events, section compression and loss of particles (“lost caps”), contribute toz-axis distortion and bias in optical disector counting
4. Tissue shrinkage and unbiased stereological estimation of particle number and size*
5. The new stereological tools: Disector, fractionator, nucleator and point sampled intercepts and their use in pathological research and diagnosis
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